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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,254)
Mouse Secondary Antibodies (14,502)
Rabbit Secondary Antibodies (6,318)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,238)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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2,506 – 2,520 of 72,091 results
2,506

RY655 Rat Anti-Mouse IgM, BD OptiBuild™

Rat Monoclonal Secondary Antibody

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
Target Species Mouse
Host Species Rat
Conjugate RY655
Applications Flow Cytometry
Form Liquid
Isotype IgG2a κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Gene Alias Igh-M; Igm; Immunoglobulin M heavy chain
Product Type Antibody
Gene ID (Entrez) 16019
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen Not reported
Primary or Secondary Secondary
Clone II/41
2,507

Rat Anti-Mouse IgG2A PE-conjugated Antibody, R&D Systems™
GREENER_CHOICE SDP

Rat Monoclonal Antibody has been used in 2 publications

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Mouse
Host Species Rat
Conjugate R-PE
Applications Flow Cytometry
Form Purified
Isotype IgG1
Antigen IgG2a
Regulatory Status RUO
Purification Method Protein A or G purified from hybridoma culture supernatant
Dilution Flow Cytometry 10 uL/10^6 cells
Formulation Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
Immunogen Cocktail of mouse IgG 1 and IgG 2A
Classification Monoclonal
Primary or Secondary Secondary
Test Specificity The specificity of this antibody was confirmed by direct ELISA on mouse IgG 2A monoclonal antibodies, and it does not cross-react with IgG 1 IgG 2B IgG 3 IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG 2A isotype, and does not detect primary antibodies of mouse IgG 1 IgG 2B or IgG 3 class.
Clone 344701
2,508

Invitrogen™ Rat anti-Mouse IgE (Heavy chain) Secondary Antibody, FITC

Rat Monoclonal Secondary Antibody

Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Target Species Mouse
Host Species Rat
Conjugate FITC
Applications Flow Cytometry
Form Liquid
Isotype IgG1
Concentration 1 mg/mL
Antigen Mouse IgE (Heavy chain)
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 50% glycerol and 0.1% sodium azide
Immunogen Purified IgE from BALB/c mice.
Classification Monoclonal
Primary or Secondary Secondary
Clone LO-ME-3
2,509

Chicken anti-Mouse IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Mouse
Host Species Chicken
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins
2,510

Chicken anti-Mouse IgG (H+L) Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Mouse
Host Species Chicken
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:2000 - 1:20000, ELISA 1:2000 - 1:20000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against human and rabbit IgG/serum proteins.
2,511

Chicken anti-Rabbit IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Chicken
Conjugate Janelia Fluor 525
Applications Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunoassay,Immunomicroscopy,Chemiluminescence Immunoassay (CIA)
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Chicken anti-Rabbit IgG (H+L) Secondary Antibody was developed against purified rabbit IgG (H&L).
Primary or Secondary Secondary
2,512

Invitrogen™ Chicken anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP

Chicken Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Rat
Host Species Chicken
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Form Lyophilized
Isotype IgY
Concentration 1 mg/mL
Antigen Rat IgG (H+L) Cross-Adsorbed
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.1% ProClin 150; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
2,513

Chicken anti-Goat IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Chicken
Conjugate FITC
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20-1:2000, Immunomicroscopy 1:20-1:2000
Classification Polyclonal
Immunogen Purified Goat IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on goat IgG and light chains on all goat immunoglobulins
2,514

Chicken anti-Rat IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Rat
Host Species Chicken
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.5mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against human and rabbit IgG and serum proteins.
2,515

Chicken anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Chicken
Conjugate DyLight 350
Applications ChIP Assay,Dot Blot,ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Primary or Secondary Secondary
2,516

Chicken anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Chicken
Conjugate DyLight 594
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunocytochemistry/Immunofluorescence, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins
2,517

Chicken anti-Rabbit IgG (H+L) Secondary Antibody, PE, Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Chicken
Conjugate PE
Applications ELISA,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Chicken anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified rabbit IgG (H&L).
Primary or Secondary Secondary
2,518

Chicken anti-Rat IgG (H+L) Secondary Antibody, PE, Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rat
Host Species Chicken
Conjugate PE
Applications ELISA,Immunoassay,Immunodiffusion,Immunohistochemistry,Immunoprecipitation,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Chicken anti-Rat IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified rat IgG (H&L).
Primary or Secondary Secondary
2,519

Chicken anti-Rat IgG (H+L) Secondary Antibody, DyLight 550 (Pre-adsorbed), Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rat
Host Species Chicken
Conjugate DyLight 550
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rat IgG (H&L)
Reconstitution Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against human and rabbit IgG and serum proteins.
2,520

Chicken anti-Human IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Chicken Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Chicken
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Dot Blot
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Human IgG whole molecule
Primary or Secondary Secondary